Analysis Note

Positive ControlUV-treated HEK293 cells or sorbitol-treated PC12 cells

Application

Immunoblotting (1:1000)

General description

Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵ in uv-treated HEK293 cells.

This PhosphoDetect™ Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.Dong, C., et al. 2001. J. Clin. Immunol.21, 253.Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.Tong, T., et al. 2001. Exp. Cell Res.269, 64.Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.Basu, S., et al. 1998. Oncogene17, 3277.

Packaging

100 µL in Plastic ampoule

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)